{"id":854,"date":"2014-09-02T13:43:19","date_gmt":"2014-09-02T17:43:19","guid":{"rendered":"\/\/phytochemia.com\/?p=854"},"modified":"2014-09-02T13:43:19","modified_gmt":"2014-09-02T17:43:19","slug":"publication-of-a-method-for-analysis-of-volatile-compounds-present-in-the-plant-material","status":"publish","type":"articles-de-blogue","link":"https:\/\/phytochemia.com\/en\/articles-de-blogue\/publication-of-a-method-for-analysis-of-volatile-compounds-present-in-the-plant-material\/","title":{"rendered":"Publication of a Method for Analysis of Volatile Compounds Present in the Plant Material"},"content":{"rendered":"

Laurie<\/span> Caron –<\/span> Summary<\/span> of articles<\/span><\/p>\n

During my<\/span> graduate studies<\/span> in<\/span> natural products chemistry<\/span> at the University<\/span> of Quebec at<\/span> Chicoutimi<\/span> (UQAC<\/span>), I had the opportunity to<\/span> publish a few<\/span> articles related to<\/span> the topic<\/span> of my research.<\/span> Today,<\/span> I present the<\/span> first<\/span> of them, it<\/span> deals with a<\/span> new method for<\/span> analysis of volatile<\/span> compounds<\/span> from<\/span> plant material<\/span>:<\/p>\n

“<\/span>Volatile compounds<\/span> in the<\/span> foliage<\/span> of balsam fir<\/span> Analyzed by<\/span> static<\/span> headspace<\/span> gas chromatography<\/span> (HS-<\/span>GC): An example of<\/span> the spruce budworm<\/span> defoliation<\/span> effect<\/span> in the boreal forest<\/span> of Quebec<\/span>, Canada”<\/span> published in the journal<\/span> Michrochemical<\/span> in 2013.<\/span><\/p>\n

Using<\/span> the traditional method<\/span> of analysis by<\/span> GC-<\/span>MS analysis of volatile compounds<\/span> during my<\/span> study would have been<\/span> a tedious process<\/span> of<\/span> manipulation<\/span> in the laboratory.<\/span> This method<\/span> requires<\/span> a<\/span> first step of extracting the<\/span> compounds<\/span> of interest<\/span> and then <\/span>the<\/span>\u00a0analysis of them<\/span>. Having<\/span> so<\/span> many samples<\/span> to<\/span> analyze<\/span> for my research<\/span> (about 200<\/span> for this component)<\/span> the development<\/span> of this method by<\/span> HS-<\/span>GC (<\/span>Static Headspace<\/span> Gas<\/span> Chromatography)<\/span> was<\/span> necessary.<\/span> My<\/span> Master Degree was<\/span>\u00a0already<\/span> divided into three<\/span> separate<\/span> components<\/span> analysis<\/span> and just<\/span> by itself<\/span>, it could have been<\/span> the sole object of<\/span> my<\/span> memory.<\/span><\/p>\n

The<\/span> HS-<\/span>GC method<\/span> differs from the one<\/span>\u00a0normally<\/span> used<\/span> (<\/span>by GC<\/span>-MS) by<\/span> eliminating the<\/span> step of extracting the<\/span> volatile compounds<\/span> and the addition of<\/span> a step of<\/span> preheating the<\/span> sample<\/span>. This preheating<\/span> allows<\/span> volatile compounds<\/span> to break free<\/span> of the initial matrix<\/span> and concentrate<\/span> in the headspace<\/span> of the bottle<\/span> containing the sample.<\/span> Then, the<\/span> principles of analysis<\/span> of the compounds<\/span> are<\/span> the same<\/span> as gas chromatography<\/span>, they are\u00a0explained<\/span> in a post<\/span> previously<\/span> done by my<\/span> colleague<\/span>: Analyses by<\/span> GC<\/span> – Part<\/span> I. Principles<\/span> of separation.<\/span><\/p>\n

The<\/span> HS-<\/span>GC method<\/span> has<\/span> saved me<\/span> a lot of time<\/span> and ultimately, get<\/span>\u00a0a reliable analysis<\/span>. For my<\/span> study<\/span>, the interest compounds<\/span> were<\/span> monoterpenes<\/span> and<\/span> sesquiterpenes.<\/span> They are a\u00a0major<\/span> part<\/span> of\u00a0essential oils<\/span> and<\/span> known to be<\/span> the defense\u00a0of<\/span> the plants. <\/span>These<\/span> are already<\/span> well known<\/span> and<\/span>\u00a0listed in<\/span> the litterature and several<\/span> databases<\/span>, it was possible for me to<\/span> easily<\/span> identify<\/span>\u00a0each<\/span> compound present in<\/span> my samples<\/span> by their<\/span> retention index<\/span>.<\/p>\n

The difficulty in<\/span> applying the method<\/span> lay in finding<\/span>\u00a0the right amount of<\/span> sample<\/span> to include in the bottles\u00a0and then<\/span>\u00a0the proper<\/span> warm-up time<\/span> so that<\/span>\u00a0the<\/span> GC<\/span> column<\/span> would not get saturated<\/span> by<\/span> volatiles.<\/span> Once these are<\/span> found<\/span>, it was necessary to make sure that<\/span> the method could<\/span>\u00a0provide<\/span> more than an alternative<\/span> to<\/span>\u00a0quickly identify the volatile compounds in <\/span>a<\/span> plant sample<\/span>, but<\/span> also be able<\/span> to quantify these<\/span> compounds.<\/span><\/p>\n


\n
\"Figure
Figure 1:<\/strong> Samples of balsam fir needles was analyzed by HS-GC<\/span><\/figcaption><\/figure>
\n<\/center><\/figure>\n

 <\/p>\n

For quantification<\/span> of<\/span> specific<\/span> molecules<\/span> such as<\/span> \u03b2<\/span>–<\/span>phellandrene (<\/span>monoterpene), an internal<\/span> standard<\/span> (<\/span>geraniol) and a commercial<\/span> standard<\/span> (<\/span>for the preparation of<\/span> a calibration curve<\/span>) were used<\/span>. Furthermore<\/span>, to ensure<\/span> the<\/span> stability of the method<\/span>, each sample was<\/span> prepared and analyzed<\/span> in triplicate.<\/span> The injection of<\/span> a single sample<\/span> several times<\/span> could not<\/span> be<\/span> done due to<\/span> the preheating step<\/span> of<\/span> the<\/span> samples<\/span>. Similar results<\/span> have been<\/span> obtained, it<\/span>\u00a0suggest that<\/span> the method<\/span> seems to<\/span> be as reliable as<\/span> those<\/span> traditionally used<\/span>.<\/p>\n

In addition,<\/span> some preliminary results<\/span> of my research on<\/span> the influence of<\/span> spruce<\/span> budworm<\/span> (SBW<\/span>) (Choristoneura<\/span> fumiferana<\/span> Clemens<\/span>) on the chemical composition<\/span> of balsam fir<\/span> have also<\/span> been included<\/span> in this article.<\/span> These<\/span> data demonstrated<\/span> that<\/span> among other<\/span> defoliation,<\/span> this conifer<\/span> needles<\/span> in the wild<\/span> had an impact on<\/span> the production of the<\/span>\u00a0defensive compounds<\/span> (<\/span>monoterpenes, sesquiterpenes<\/span>, etc). In connection with these<\/span> results<\/span>, the hypothesis that an<\/span> increased production<\/span> of<\/span> \u03b2<\/span>–<\/span>phellandrene (<\/span>monoterpene) by<\/span> fir needles<\/span> during<\/span> severe<\/span> defoliation<\/span> in the period<\/span> of\u00a0June<\/span> was also<\/span> raised.<\/span><\/p>\n","protected":false},"template":"","categories-darticle":[37],"class_list":["post-854","articles-de-blogue","type-articles-de-blogue","status-publish","hentry","categories-darticle-article-summaries"],"yoast_head":"\nPublication of a Method for Analysis of Volatile Compounds Present in the Plant Material - PhytoChemia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/phytochemia.com\/en\/articles-de-blogue\/publication-of-a-method-for-analysis-of-volatile-compounds-present-in-the-plant-material\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Publication of a Method for Analysis of Volatile Compounds Present in the Plant Material - PhytoChemia\" \/>\n<meta property=\"og:description\" content=\"Laurie Caron – Summary of articles During my graduate studies in natural products chemistry at the University of Quebec at Chicoutimi (UQAC), I had the opportunity to publish a few articles related to the topic of my research. 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